Enzyme reference materials: their place in diagnostic enzyrnology

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Enzyme reference materials: their place in diagnostic enzyrnology

Estimations of the activities of various enzymes in clinical samples are routine tasks for clinical chemists. Most of this work is done by automatic analysis. The reference ranges against which patients' results are interpreted, however, have generally been defined in terms of manual methods and the conditions of a manual method cannot be reproduced in automated systems. This paper describes th...

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A discussion of enzyme reference materials: applications and specifications.

Clinical laboratories estimating enzyme activity in serum are using commercial lyophilized sera for four major purposes. These uses-as a standard and for intermethod, intramethod, and precision control-are segregated, and specifications for each deployment are examined in terms of requirements for the enzyme material: freedom from interfering or indicator enzymes and chromogens; high specific a...

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Polymer materials for enzyme immobilization and their application in bioreactors.

Enzymatic catalysis has been pursued extensively in a wide range of important chemical processes for their unparalleled selectivity and mild reaction conditions. However, enzymes are usually costly and easy to inactivate in their free forms. Immobilization is the key to optimizing the in-service performance of an enzyme in industrial processes, particularly in the field of non-aqueous phase cat...

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Local Diagnostic Reference Levels for Some Common Diagnostic X-Ray Examinations In Sabzevar County of Iran

Introduction: Diagnostic reference level (DRL) is a useful tool for the promotion of optimization. The national DRLs (NDRLs) are useful, brief, and robust guidelines for optimizing radiation protection in a country. The aim of this study was to extract the local DRLs (LDRLs) for some common radiologic examination in Sabzevar county. Materials and Methods: There are eight radiology departments ...

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Production and certification of secondary enzyme reference materials (ERMs). Part 1: Preparation of the sera and some of their properties.

We produced three batches of a human-serum-based enzyme reference material (ERM) enriched with human aspartate aminotransferase (EC 2.6.1.1), alanine aminotransferase (EC 2.6.1.2), creatine kinase (EC 2.7.3.2), and lactate dehydrogenase (EC 1.1.1.27). The added enzymes were not exhaustively purified; thus the final ERMs contained some enzymes as contaminants, of which only glutamate dehydrogena...

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ژورنال

عنوان ژورنال: Journal of Automatic Chemistry

سال: 1994

ISSN: 0142-0453

DOI: 10.1155/s1463924694000064